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Background and purpose:Major repair genes that affect the tumor genetic susceptibility exists in repair pathways base excision repair (BER) approach, X-ray repair cross complementing group 1(XRCC1) gene, respectively is the core of BER pathway. At home and abroad in recent years, has carried out many studies of genetic polymorphism and laryngeal cancer susceptibility. Researching on the base excision repair (BER) pathway of DNA repair geneXRCC1 bases mononuclear nucleotide polymorphism and the relationship between different ethnic groups laryngeal cancer susceptibility in xinjiang.Methods:A case-control study was performed on 58 patient with laryngeal squamous cell carcinoma and 120 random healthy control group. Multiplex SNaPshot technic was used to detect DNA base excision repair geneXRCC1 Gln632Gln (rs3547), Arg399Gln (rs25487), Arg280His (rs25489), Arg194Trp (rs1799782) loci single nucleotide polymorphism distribution in the case group and normal control group.Results:Three sites of the rest of the cases ofXRCC1 Gln632Gln (rs3547) C/T (hybrid) and T/T (mutant) genotype, Arg399Gln (rs25487) C/T (hybrid) and T/T (mutant) genotype, Arg194Trp (rs1799782) G/A (hybrid) and A/A (mutant) genotype is notably higher than that of control group (P<0.01). Gln632Gln (rs3547) C/T (hybrid) and T/T (mutant) genotype, Arg399Gln (rs25487) C/T (hybrid) and T/T (mutant) genotype, Arg194Trp (rs1799782) G/A (hybrid) and A/A (mutant) genotype ratio is signiifcantly higher than control group (P<0.05) in cases of Han,Uygur and Kazakh nations, carrying (rs3547) C/T and T/T genotype, (rs25487) C/T and T/T genotype, (rs1799782) G/A and A/A genotype individual risk of laryngeal squamous cell carcinoma are added to the 0.96, 1.74 and 1.39 times; 1.47, 1.32 and 0.77 times; 1.49, 1.51 and 1.56 times thanXRCC1 (rs3547) C/C genotype, (rs25487) C/C genotype, (rs1799782) G/G genotype.Conclusion:In the 3 nations,XRCC1 Gln632Gln, Arg399Gln, Arg280His and Arg194Trp loci polymorphism may be associated with laryngeal cancer genetic and there are differences,XRCC1 Gln632Gln, Arg399Gln, Arg194Trp locus mutation will lead to an elevated risk of throat cancer.XRCC1 Arg280His locus mutation has no statistically signiifcant difference with the onset of throat cancer, may have nothing to do with the onset of laryngeal cancer on the site of mutation.
ABSTRACT
OBJECTIVE@#To study genetic polymorphism of XRCC1 Arg399Gln and the laryngeal cancer risk.@*METHOD@#A case-control study was performed on 60 patients with laryngeal squamous cell carcinoma and 120 random healthy control group. The two groups were matched by sex and age. Multiplex SNaPshot technic was used to explore polymorphism of DNA repair gene XRCC1 Arg399Gln in distribution of patient with laryngeal squamous cell carcinoma and normal control.@*RESULT@#The frequency of XRCC1c. 399Arg/Gln+Gln/Gln genotypes in the case group was higher than that in the control group (P Gln might lead to a increased risk of laryngeal cancer.
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Case-Control Studies , China , Epidemiology , DNA Repair , DNA-Binding Proteins , Genetics , Ethnicity , Genetics , Laryngeal Neoplasms , Epidemiology , Genetics , Polymorphism, Single Nucleotide , X-ray Repair Cross Complementing Protein 1ABSTRACT
OBJECTIVE@#To understand the basic situation and development of knowledge structure and ability of master degree of Otolaryngology Head and Neck Surgery in Xinjiang region in order to provide reference to further improve the quality of postgraduate students.@*METHOD@#Fourty-six papers of Otolaryngology master degree thesis were reviewed at randomly in terms of types, subject selection ranges as well as statistical methods during 1998-2009 in Xinjiang region in order to analyze and explore its advantages and characteristics and suggest a solution for its disadvantages.@*RESULT@#In 46 degree thesis, nine of them are scientific dissertations accounting for 19.57%, 37 are clinical professional degree thesis, accounting for 80.43%. Five are Experimental research papers, 30 are clinical research papers, 10 are clinical and experimental research papers, 1 of them is experimental epidemiology research paper; in this study, the kinds of diseases including every subject of ENT, various statistical methods are involved; references are 37.46 in average, 19.55 of them are foreign literatures references in nearly 5 years are 13.57; four ethnic groups are exist in postgraduate students with high teaching professional level of tutors.@*CONCLUSION@#The clinical research should be focused in order to further research on ENT common diseases, the application of advanced research methods, the full application of the latest literature, tutors with high-level, training of students of various nationalities, basic research needs to be innovative and should be focus the subject characteristics, to avoid excessive duplication of research.
Subject(s)
Academic Dissertations as Topic , China , Otolaryngology , EducationABSTRACT
Objective: The Beagle dog is suitable for biomedical researches due to its stable biological characteristics.This study aimed to investigate the anatomical application value of the Beagle dog in the reconstruction of hypopharyngeal and cervical esophagus.Methods: Ten Beagle dogs selected for the reconstruction of hypopharyngeal and cercvical esophagus with free jejunal flap transplantation underwent detailed local anatomy of the neck muscles,blood vessels and nerves.Results: The Beagle dogs had strong neck muscles,superficial and thin jugular veins,clear arterial branches,firm esophagus endomembrane,and strong antitrauma capacity.Conclusion: Anatomic characteristics of the neck of the Beagle dog has special application value and clinical significance for further studies on neck tissue defect reconstruction and organ transplantation.